Wednesday, May 21, 2014

Quick Observation 1 - Δhfq growth rate

I did my first set of transformations today using KW20 and Δhfq (RR3187) strains of H. influenzae. While waiting for the cells to divide I noticed the Δhfq broth took a lot longer to grow...


Turns out this is one of the pleiotropic characteristics of an hfq mutant.


Hfq is further underlined by an interruption of its gene, which... causes pronounced pleiotropic phenotypes such as decreased growth rate, increased cell length, and sensitivity to UV light.

 I'll just have to remember to plan for an extra half hour incubation time when working with Δhfq.

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