I did my first set of transformations today using KW20 and
Δhfq (RR3187) strains of H. influenzae. While waiting for the cells to divide I noticed the Δhfq broth took a lot longer to grow...
Turns out this is one of the pleiotropic characteristics of an hfq mutant.
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 Hfq is further underlined by an interruption of its
gene, which... causes
pronounced pleiotropic phenotypes such as decreased growth rate,
increased cell length, and sensitivity to UV light. |
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I'll just have to remember to plan for an extra half hour incubation time when working with
Δhfq.
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